一种新的对虾病原菌——雷氏普罗威登斯菌

本文从患败血病的中国对虾体内分离到多株细菌，其中三株经人工感染证实为病原菌，又经４０多项形态、生理、生化特性测试，鉴定为雷氏普罗威登斯菌（Ｐｒｏｖｉｄｅｎｃｉａｒｅｔｇｅｒｉ），其主要特性为杆状，革兰氏阴性，周生鞭毛，运动，无自然游动现象，兼性厌氧，苯丙氨酸脱氨酶、柠檬酸盐利用、吲哚、甲基红反应均阳性，发酵甘露醇、肌醇、甘露糖产酸。赖氨酸、鸟氨酸脱羧酶、精氨酸双水解酶、ＶＰ反应均阴性。生长最适温度、ＮａＣｌ、ｐＨ范围分别为２５℃—３０℃、０５％—１５％、７０—９０。对羧苄青霉素、先锋霉素５号、氯霉素、ＳＭＺ＋ＴＭＰ、氟哌酸、链霉素等极为敏感。雷氏普罗威登斯菌引起对虾败血病在国内外尚属首次报道。     

不同密度的羊草种群结构及其光照分布的研究

本文报道了耕翻后自然更新的羊草种群不同密度条件下垂直结构及其光照分布特征。结果表明，地上生物量和叶面积指数均以种群中部最大，向上或向下皆逐渐减小。在三种密度中，生物量，叶面积指数，株高，茎高和叶片含水量均随着密度的增大而升高。比叶重和单株绿叶数度增加而降低。     

猕猴桃16个优良品种（变种）果实主要经济性状的研究

猕猴桃１６个优良品种（变种）果实主要经济性状研究表明，地处金衡盆地东南边缘的武义县，适宜种植和开发布鲁诺、ＫＨ－５两个优良品种，具经济性状好、果大、风味浓、丰产、供应期长等特点。     

免疫低聚糖对中国对虾的免疫效用研究

为证实免疫低聚糖(D-氨基葡聚寡糖,D-Amino-Oligosacellaride)对中国对虾(Penaeus chinensis)的免疫增强作用,进行了不同剂量的注射及饲料投喂实验.结果表明,注射免疫低聚糖可显著增强中国对虾酸性磷酸酶和溶茵酶等血清免疫指标,添加于饲料中投喂可以提高对虾成活率,降低饵料系数,且饲料中添加量≥3‰的效果较佳.     

不同熟期荔枝品种的枝梢生长节奏的初步研究

本试验以3-4年生的早熟（‘三月红’）、中熟（‘妃子笑’）、晚熟（‘糯米糍’）及特迟熟（‘马贵荔’）荔枝品种为试材，比较不同成熟期荔枝品种周年的枝梢生长节奏及新叶发育过程中叶绿素指（SPAD）数动态。结果表明：各品种枝梢伸长生长具有一定差异，但都具有一定的节奏性，而枝梢增粗生长周年持续进行，无明显节奏特征；叶绿素指数达到最高值的80%时增加缓慢，因此叶绿素指数达最高值的80%时作为枝梢成熟的标准，用来确定枝梢成熟的时间。     

微卫星技术对黄、渤海海域7个不同地理群体中国对虾的遗传结构和遗传分化研究

采用微卫星DNA技术对黄、渤海海域7个不同地理群体的中国对虾进行了遗传结构和遗传分化研究.7个地理群体分别来自辽东湾(LD)、渤海湾(BH)、海州湾 (HZ)、乳山湾 (RS)、海洋岛 (HYD)、朝鲜半岛西海岸(KW)以及朝鲜半岛南海岸(KS).7对多态性良好的微卫星引物共检测到109个等位基因,群体平均期望杂合度(He)范围为0.810～0.864.49个群体位点中,只有15个符合Hardy-Weinberg平衡.UPGMA聚类分析显示,各地理群体亲缘关系与地理位置关系密切.根据各地理群体间遗传距离及AMOVA遗传分化检验结果,可将中国对虾分为3个独立种群,分别为中国沿岸黄、渤海群体、朝鲜半岛西海岸群体及朝鲜半岛南海群体;其中中国沿岸黄、渤海群体内部也发生了一定程度的遗传分化,但是否达到种群水平还有待于进一步验证.     

Preparation and Characterization of Protein Hydrolysates From Little Loligo Squid (Uroteuthis chinensis)

Research on the little loligo squid (Uroteuthis chinensis) protein hydrolyzed by papain and Flavourzyme and characterization of hydrolysates was carried out in this article. The optimum hydrolysis conditions of papain were obtained by orthogonal experiments, and the influence factors on the degree of hydrolysis such as enzyme/substrate ratio, hydrolysis time, pH, and temperature were investigated. The effect of Flavourzyme on the degree of hydrolysis was also studied. The characteristics of freeze-dried hydrolysates such as yield, moisture, nitrogen recovery, molecular mass distribution, and amino acid composition were determined. The results showed that papain and Flavourzyme could hydrolyze the little loligo squid protein efficiently, and the degree of hydrolysis was up to 25.82% under the optimum hydrolysis conditions. The average molecular mass of hydrolysates obtained with papain and Flavourzyme was 1,246 Da, and the low molecular mass peptides were the main fraction of hydrolysates. The yield, moisture, and nitrogen recovery of freeze-dried hydrolysates was 19.8, 4.4, and 74.2%, respectively. Compared with suggested patterns of amino acid requirements by the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), the hydrolysates of the little loligo squid had high nutritional value, and was a potential nutritious supplement used in various food products.     

Comparison of defence ability against the white spot syndrome virus between Fenneropenaeus chinensis and Marsupenaeus japonicus

The defence ability of Fenneropenaeus chinensis and Marsupenaeus japonicus against the intrusion of the white spot syndrome virus (WSSV) was compared after injecting WSSV intramuscularly by recording cumulative mortality, diagnosing the virus and examining variations in immunological parameters including the total haemocyte counts (THCs), phagocytic percentage (PP), plasma protein concentration (PPC), phenoloxidase (PO) and nitric oxide synthase (NOS) activity. The results showed that the variations in the immunological parameters of F. chinensis and M. japonicus showed similar trends. The THCs of the two species decreased significantly postchallenge of WSSV. The virus was detected at 78 h in M. japonicus and at 42 h in F. chinensis after infection, which was in correlation with the accumulative mortality, and the variations in PO, PP, NOS and PPC in the two species. All shrimps of F. chinensis in the mortality experiment died within 66 h, much more quickly than M. japonicus, whose cumulative mortality reached 100% after 198 h. In conclusion, the immune system of M. japonicus has a stronger resistant ability to antagonize and endure the invasion of WSSV than that of F. chinensis.     

石竹花芽发生与内源多胺含量的关系

以石竹叶片为外植体，诱导愈伤组织、营养芽、花芽的培养基分别为ＭＳ＋ＺＴ２．５ｍｇ／Ｌ＋２，４－Ｄ０．３ｍｇ／Ｌ；ＭＳ＋ＺＴ１ｍｇ／Ｌ＋ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ；ＭＳ＋ＩＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．３ｍｇ／Ｌ。在石竹离体叶花芽形成过程中，内源多胺含量呈不同的变化趋势，腐胺、亚精胺含量上升，而二丙胺、精胺含量下降，表明多胺与石竹离体叶花芽分化有明显的内在联系。     

闽南沿海沙质地区希蒙得木的引种

引种试验表明:希蒙得木在闽南沿海温暖潮湿的沙质地区不论原种或子代都生长良好、能自然开花结果,物候期及生长规律表达稳定,种子含油量及品质与原产地基本一致,引种试验成功;但由于病虫害等原因,希蒙得木在引种点无人为管理的情况下无法自然生存。沙地良好的通透性和林地良好的排水性是希蒙得木能承受当地高达1325mm年降雨量的主要原因,也是希蒙得木在闽南沿海沙质地区引种成功的主导因子;希蒙得木在引种点的耐旱性不强,旱季浇水有助于植株生长。